Influence of Hypoxia, Storage Period, or Temperature on the Taste of Broccoli Florets

Authors:Demi Armstrong


In the present study, the paper aimed to maintain sulforaphane concentration by cold storage or by freezing, either of which could be expected to depress the reactions to reduce causing sulforaphane loss and off-odors such as ethanol after 2 days storage. Also, changes in the taste of broccoli florets over time at different storage temperatures were investigated by objective measurement. This experiment was conducted to confirm the influence of hypoxia, storage period, or temperature on the taste of broccoli florets. Each floret was sealed in a separate pouch. All pouches were stored in incubators at 20℃, 1℃ and -20℃. The sulforaphane concentration and objective taste values were measured in four and three pouches, respectively, from each treatment group at 0 day, 2 days, and 6 days of storage. The results showed that sulforaphane concentration of broccoli florets kept for 2 days under hypoxia increased and investigated what effect subsequent storage time and temperature over 4 days had on the sulforaphane concentration and taste of the broccoli florets. After storage at -20℃ (frozen) for 4 days, the sulforaphane concentration was not significantly reduced compared with the maximum level on day 2. Meanwhile, this concentration was not significantly higher than those at 20℃ or 1℃ on the same day. However, even when stored at -20℃, changes in taste (objectively measured using an electronic tongue) were not prevented, as demonstrated by PC analysis. Freezing may be a desirable method for maintaining sulforaphane levels in broccoli florets, and that future research could focus on an effective method for preventing changes in taste during storage.

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Eason, J.R., D. Patel, D. Ryan, B. Page, D. Hedderley, L. Watson and P. West. 2007. Controlled atmosphere treatment of broccoli after harvest delays senescence and induces the expression of novel BoCAR genes. Plant Physiology and Biochemistry, 45 (6-7): 445-456.

Esturk, O., Z. Ayhan and T. Gokkurt. 2014. Production and application of active packaging film with ethylene adsorber to increase the shelf life of broccoli (Brassica oleracea l. Var. Italica). Packaging Technology and Science, 27 (3): 179-191.

Fan, X.T. and J.P. Mattheis. 2000. Yellowing of broccoli in storage is reduced by 1-methylcyclopropene. Hortscience, 35 (5): 885-887.

Funamoto, Y., N. Yamauchi, T. Shigenaga and M. Shigyo. 2002.

Effects of heat treatment on chlorophyll degrading enzymes in stored broccoli (Brassica oleracea L.). Postharvest Biology and Technology, 24 (PII S0925-5214(01)00135-12): 163-170.

Kasim, R. and M.U. Kasim. 2007. Inhibition of yellowing in Brussels sprouts (B. Oleraceae var. Gemmifera) and broccoli (B. Oleraceae var. Italica) using light during storage. Journal of Food Agriculture & Environment, 5 (3-4): 126-130.

Lemoine, M.L., A.R. Chaves and G.A. Martinez. 2010. Influence of combined hot air and UV-C treatment on the antioxidant system of minimally processed broccoli (Brassica oleracea L. Var. Italica). Lwt-Food Science and Technology, 43 (9): 1313-1319.

Murcia, M.A., B. Lopez-Ayerra and F. Garcia-Carmona. 1999. Effect of processing methods and different blanching times on broccoli: Proximate composition and fatty acids. Food Science and Technology-Lebensmittel-Wissenschaft & Technologie, 32 (4): 238-243.

Pellegrini, N., E. Chiavaro, C. Gardana, T. Mazzeo, D. Contino, M. Gallo, P. Riso, V. Fogliano and M. Porrini. 2010. Effect of different cooking methods on color, phytochemical concentration, and antioxidant capacity of raw and frozen brassica vegetables. Journal of Agricultural and Food Chemistry, 58 (7): 4310-4321.

Sanz, S., C. Olarte, F. Ayala and J.F. Echavarri. 2008. The response to lighting of minimally processed chard: Influence on its shelf life. Journal of the Science of Food and Agriculture, 88 (9): 1622-1631.

Schouten, R.E., X. Zhang, J.A. Verschoor, E.C. Otma, L.M.M. Tijskens and O. van Kooten. 2009. Development of colour of broccoli heads as affected by controlled atmosphere storage and temperature. Postharvest Biology and Technology, 51 (1): 27-35.


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