Estimating Genetic Similarity in Sesame Accessions Based on Morphological, Agronomic, and Molecular Descriptors

Authors:Deacon Gilliam, Jasmin Youn

Abstract


Grouping methods are the most commonly used, as long as they can add criteria that show similarity in behavior patterns related to character sets according to certain criteria. In this study, we used a multivariate method to estimate the genetic similarity of sesame germplasm based on morphology, agronomy and molecular descriptors. For the analysis, 36 sesame deposits in the Embrapa genebank were used. Five plants (morphological variables) selected in the center row after germination and about 60 days out of about 90 days (agronomic variables) were evaluated. As shown in the results, molecular markers and morphological agronomic markers can effectively estimate the variability between sesame germplasm, and can identify the most similar markers (ABG 591, ABG 616, ABG 649, ABG 141, and ABG 688) and have the largest differences (ABG 648 and ABG 200) genotypes.

Full Text:

PDF

References


Ali, G.M., S. Yasumoto and M. Seki-Katsuta. 2007. Assessment of genetic diversity in sesame (Sesamum indicum L.) detected by Amplified Fragment Length Polymorphism markers. Electronic Journal of Biotechnology, 10 (1): 12-23.

Badri, J., V. Yepuri, A. Ghanta, S. Siva and E.A. Siddiq. 2014. Development of microsatellite markers in sesame (Sesamum indicum L.). Turkish Journal of Agriculture and Forestry, 38 (5): 603-614.

Ercan, A.G., M. Taskin and K. Turgut. 2004. Analysis of genetic diversity in Turkish sesame (Sesamum indicum L.) populations using RAPD markers. Genetic Resources and Crop Evolution, 51 (6): 599-607.

Laurentin, H.E. and P. Karlovsky. 2006. Genetic relationship and diversity in a sesame (Sesamum indicum L.) germplasm collection using amplified fragment length polymorphism (AFLP). Bmc Genetics, 7 (10).

Maluszynski, M., I. Szarejko, P. Barriga and A. Balcerzyk. 2001. Heterosis in crop mutant crosses and production of high yielding lines using doubled haploid systems. Euphytica, 120 (3): 387-398.

Sharma, S.N., V. Kumar and S. Mathur. 2009. Comparative analysis of RAPD and ISSR markers for characterization of sesame (Sesamum indicum l) genotypes. Journal of Plant Biochemistry and Biotechnology, 18 (1): 37-43.

Surapaneni, M., V. Yepuri, L.R. Vemireddy, A. Ghanta and E.A. Siddiq. 2014. Development and characterization of microsatellite markers in Indian sesame (Sesamum indicum L.). Molecular Breeding, 34 (3): 1185-1200.

Uncu, A.O., V. Gultekin, J. Allmer, A. Frary and S. Doganlar. 2015. Genomic simple sequence repeat markers reveal patterns of genetic relatedness and diversity in sesame. Plant Genome, 8 (2).

Wei, L., H. Miao, F. Xu, J. Kong and H. Zhang. 2017. Chinese sesame cultivars, DNA fingerprinting, and two-dimensional barcodes using single nucleotide polymorphisms, insertions or deletions, and simple sequence repeat markers. Crop Science, 57 (4): 1941-1947.

Wei, W., X. Qi, L. Wang, Y. Zhang, W. Hua, D. Li, H. Lv and X. Zhang. 2011. Characterization of the sesame (Sesamum indicum L.) global transcriptome using Illumina paired-end sequencing and development of EST-SSR markers. Bmc Genomics, 12 (451).

Zhang, Y., X. Zhang, W. Hua, L. Wang and Z. Che. 2010. Analysis of genetic diversity among indigenous landraces from sesame (Sesamum indicum L.) core collection in China as revealed by SRAP and SSR markers. Genes & Genomics, 32 (3): 207-215.


Refbacks

  • There are currently no refbacks.