An IP-UPLC-Based Method of Determining Nitrate Ion Levels in Fresh Vegetable Juices

Authors:Hudson Martyn, Donkin Hazel


The content of nitrate ions in vegetables has become a human health problem. Accurate and rapid tissue (root, stem, or leaf stem) testing of crops is a valuable tool for determining fertilizer needs and maximizing fertilizer efficiency. There are liquid chromatography methods for measuring the content of nitrate ions in vegetables. The ion chromatography (IC) method is reliable, but each vegetable sample typically requires a longer run time (47 minutes). However, IC columns have not been developed for ultra-high-performance liquid chromatography (UPLC). On the other hand, Hiraki et al. He developed a method for separating five anionic performance liquid chromatography (HPLC) using a C30 column, although a C30 column in UPLC still requires about 18 minutes of run time. In contrast, because the UPLC method is relatively fast, IP-UPLC with a C18 column may reduce run time. Therefore, the current research focus is to develop an IP-UPLC-based method to determine the nitrate ion content in fresh vegetable juices.

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