Isolating and Identifying the Species of Cephaleuros on Rambutan Based on Its Morphology and Molecular Analysis

Authors:Davis Aaron, Kaur Casey

Abstract


Algae leaf spot is considered a minor disease in Thailand, so little research has been done on the biology, ecology, distribution, taxonomy and pathogenicity of Cephaleuros. Therefore, the purpose of this study was to isolate and identify the genus Cephaleuros based on the morphological and molecular analysis of rambutan. In August 2014, 30 rambutan leaf spot samples (n = 30) were collected. Algae were removed from the fresh leaves and observed under a stereo microscope. In this study, the morphological and molecular characteristics of organisms isolated from rambutan leaf spots were characterized. Algae of the genus Cephalosporin have been reported to cause disease in many plant species in various habitats. Algae have been found to grow under the stratum corneum of the host. The tissue below the thalamus is necrotic, and there is no necrotic spot in the thalamus. A cross section through thalli indicates that plant tissue necrosis is spreading from thalli to healthy tissue.


Full Text:

PDF

References


Brooks, F.E., F. Rindi, Y. Suto, S. Ohtani and M. Green. 2015. The trentepohliales (Ulvophyceae, Chlorophyta): An unusual algal order and its novel plant pathogen, cephaleuros. Plant Disease, 99 (6): 740-753.

Bunjongsiri, P. and A. Sunpapao. 2018. Optimal growth conditions for in vitro cultures of plant parasitic algae cephaleuros kunze ex e. M. Fries. Philippine Agricultural Scientist, 101 (1): 45-50.

Han, K., M. Park, J. Park and H. Shin. 2011. First report of algal leaf spot associated with Cephaleuros virescens on greenhouse-grown Ficus benghalensis in Korea. Australasian Plant Disease Notes, 6 (1): 72-73.

Pitaloka, M.K., V. Petcharat, S. Arikit and A. Sunpapao. 2015. Cephaleuros virescens, the cause of an algal leaf spot on Para rubber in Thailand. Australasian Plant Disease Notes, 10 (UNSP 41).

Sanahuja, G., P. Lopez, A.J. Palmateer and A.R. Chase. 2018. Red rust of neoregelia bromeliads caused by a parasitic alga cephaleuros parasiticus in florida. Plant Health Progress, 19 (1): 27-33.

Sunpapao, A., M.K. Pitaloka and S. Arikit. 2015. The genus Cephaleuros Kunze ex EMFries (Trentepohliales, Ulvophyceae) from southern Thailand. Nova Hedwigia, 101 (3-4): 451-462.

Sunpapao, A., N. Thithuan, M.K. Pitaloka and S. Arikit. 2016. Algal leaf spot of Lansium Parasiticum caused by Cephaleuros sp in Thailand. Journal of Plant Pathology, 98 (2): 369.

Sunpapao, A., P. Bunjongsiri, N. Thithuan and S. Arikit. 2017. First report of Cephaleuros virescens causing algal leaf spot of Manilkara zapota in Thailand. Plant Disease, 101 (4): 636.

Suto, Y. and S. Ohtani. 2011. Morphological features and chromosome numbers in cultures of five Cephaleuros species (Trentepohliaceae, Chlorophyta) from Japan. Phycological Research, 59 (1): 42-51.

Suto, Y. and S. Ohtani. 2013. Seasonal development of five Cephaleuros species (Trentepohliaceae, Chlorophyta) on the leaves of woody plants and the behaviors of their gametes and zoospores. Phycological Research, 61 (2): 105-115.

Wonglom, P., N. Thithuan, P. Bunjongsiri and A. Sunpapao. 2018. Plant-Parasitic Algae (Cephaleuros spp.) in Thailand, including four new records. Pacific Science, 72 (3): 363-371.

Zhu, H., Z. Hu and G. Liu. 2017. Morphology and molecular phylogeny of Trentepohliales (Chlorophyta) from China. European Journal of Phycology, 52 (3): 330-341.


Refbacks

  • There are currently no refbacks.