Developing Somaclonal Variation of Bogor Taro to Expand the Diversity of Character

Authors:Bobby Brain, Jason Stuart

Abstract


Taro (Colocasia esculenta (L.) Schott) is a monocot which is included in the Araceae Family, and one of the staple foods as a source of carbohydrate. In the framework of breeding, the diversity of traits is required, and the induction of somatic mutation is one of the technical options utilized. The aim of this study was to develop somatic asexual variation of botanic mites to expand the diversity of traits, leading to the production of new genotypes that were then propagated to meet the needs of quality seeds. Top bud culture of the botanic taro was induced by gamma ray irradiation at 0, 5, 10, 15 and 20 Gy. The results showed that γ-rays had an effect on the number of leaves, and had the greatest effect at 10 Gy dose, but had no effect on shoots. Compared with the parental, the induction of somatic clonal variation produced up to 51% diversity.


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References


Francischini, J.H.M.B., E.L. Kemper, J.B. Costa, J.R.V. Manechini and L.R. Pinto. 2017. DNA methylation in sugarcane somaclonal variants assessed through methylation-sensitive amplified polymorphism. Genetics and Molecular Research, 16 (160295852).

Malamug JJF, Yazawa S, Asahira T. 1994. Morphological variants induced from shoot tips of taro (Colocasia esculenta (L.) Schott) treated with gamma radiation. Scientia Horticulturae 58: 105-113.

Moreno-Vazquez, S., N. Larranaga, E.C. Uberhuaga, E.J. Bolacel Braga and C. Perez-Ruiz. 2014. Bacterial contamination of in vitro plant cultures: Confounding effects on somaclonal variation and detection of contamination in plant tissues. Plant Cell Tissue and Organ Culture, 119 (3): 533-541.

Seetohul S, Puchooa D, Ranghoo-Sanmukhiya VM. 2008. Genetic Improvement of Taro (Colocasia esculenta var esculenta) through in- vitro mutagenesis. UoM Res J Univ Mauritius. Special Issue 13A: 79- 89.

Shooshtari, L., M. Omidi, E. Majidi, N. Mohamadreza, R. Shamsali, A. Etminan, A. Oladzad, M. Ghorbanpour and A. Ghaderi. 2011. Assessment of somaclonal variation in Ducrosia anethifolia plants regenerated from long-term callus cultures using AFLP. Planta Medica, 77 (12): 1287.

Tripathy, S.K., A. Panda and D. Lenka. 2016. Cytological elucidation of somaclonal variation in grasspea (Lathyrus sativus L.). Legume Research, 39 (2): 177-182.

Vos P, Hogers R, Bleeker M, Reijans M, van de Lee T, Hornes M, Frijters A, Pot J,Peleman J, Kulper M, Zabeau M. 1995. AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res 23 (21): 4407- 4414.

Yasin, H., M. Usman, H. Rashid, A. Nasir, A. Sarwar and I.A. Randhawa. 2017. Guidelines for Environmental Impact Assessment of JHAL flyover and underpass project in Faisalabad. Geology, Ecology, and Landscapes, 1 (3): 205-212.


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